OrangerV1, Main, Exploration, bibRecord, 003371

Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration

Identifieur interne : 003371 ( Main/Exploration ); précédent : 003370; suivant : 003372

Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration

Auteurs : L. Pena [Espagne] ; M. Cervera [Espagne] ; J. Juarez [Espagne] ; A. Navarro [Espagne] ; J. A. Pina [Espagne] ; L. Navarro [Espagne]

Source :

Plant cell reports [ 0721-7714 ] ; 1997.

RBID : Pascal:97-0468277

Descripteurs français

Pascal (Inist)
- Plante transgénique, Transformation génétique, Agrobacterium tumefaciens, Condition opératoire, Expression génique, Culture cellulaire mixte, Citrus aurantifolia, Optimisation, Régénération, Gène indicateur, β-Glucuronidase, Végétal, Agrume.
Wicri :
- topic : Plante transgénique, Agrume.

English descriptors

KwdEn :
- Agrobacterium tumefaciens, Citrus aurantifolia, Citrus fruit, Gene expression, Genetic transformation, Mixed cell culture, Operating conditions, Optimization, Regeneration, Reporter gene, Transgenic plant, Vegetals, β-Glucuronidase.

Abstract

We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. OsbeckxPoncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We now report on the optimization of the cocultivation, regeneration and selection conditions for efficient and reliable production of transgenic lime (C. aurantifolia Swing.) plants. Improved transformation frequencies were obtained by cocultivating the explants with Agrobacterium on feeder plates. Optimum regeneration of transgenic shoots was obtained by exposing the explants to darkness for 2 weeks and by using kanamycin at 100 mg/l as selective agent. Attempts to use geneticin as selection antibiotic were not successful. Shoot tip grafting of regenerated shoots on Troyer citrange seedlings resulted in 100% successful production of transgenic plants. The presence and expression of the transferred genes in the regenerated plants was verified by β-glucuronidase histochemical and fluorimetric assays, neomycin phosphotransferase ELISA assays, PCR and Southern analyses.

Affiliations:

Espagne

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000B18
to stream PascalFrancis, to step Curation: 001390
to stream PascalFrancis, to step Checkpoint: 000B15
to stream Main, to step Merge: 003621
to stream Main, to step Curation: 003371

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration</title>
<author><name sortKey="Pena, L" sort="Pena, L" uniqKey="Pena L" first="L." last="Pena">L. Pena</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">97-0468277</idno>
<date when="1997">1997</date>
<idno type="stanalyst">PASCAL 97-0468277 INIST</idno>
<idno type="RBID">Pascal:97-0468277</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000B18</idno>
<idno type="wicri:Area/PascalFrancis/Curation">001390</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000B15</idno>
<idno type="wicri:doubleKey">0721-7714:1997:Pena L:genetic:transformation:of</idno>
<idno type="wicri:Area/Main/Merge">003621</idno>
<idno type="wicri:Area/Main/Curation">003371</idno>
<idno type="wicri:Area/Main/Exploration">003371</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration</title>
<author><name sortKey="Pena, L" sort="Pena, L" uniqKey="Pena L" first="L." last="Pena">L. Pena</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Departamento Protección Vegetal y Biotecnologia, Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial</s1>
<s2>46113-Moncada, Valencia</s2>
<s3>ESP</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Espagne</country>
<wicri:noRegion>46113-Moncada, Valencia</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant cell reports</title>
<title level="j" type="abbreviated">Plant cell rep.</title>
<idno type="ISSN">0721-7714</idno>
<imprint><date when="1997">1997</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant cell reports</title>
<title level="j" type="abbreviated">Plant cell rep.</title>
<idno type="ISSN">0721-7714</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens</term>
<term>Citrus aurantifolia</term>
<term>Citrus fruit</term>
<term>Gene expression</term>
<term>Genetic transformation</term>
<term>Mixed cell culture</term>
<term>Operating conditions</term>
<term>Optimization</term>
<term>Regeneration</term>
<term>Reporter gene</term>
<term>Transgenic plant</term>
<term>Vegetals</term>
<term>β-Glucuronidase</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Plante transgénique</term>
<term>Transformation génétique</term>
<term>Agrobacterium tumefaciens</term>
<term>Condition opératoire</term>
<term>Expression génique</term>
<term>Culture cellulaire mixte</term>
<term>Citrus aurantifolia</term>
<term>Optimisation</term>
<term>Régénération</term>
<term>Gène indicateur</term>
<term>β-Glucuronidase</term>
<term>Végétal</term>
<term>Agrume</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Plante transgénique</term>
<term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">We have previously developed procedures for the efficient production of sweet orange (Citrus sinensis L. Osbeck) and Carrizo citrange (C. sinensis L. OsbeckxPoncirus trifoliata L. Raf.) transgenic plants using an Agrobacterium tumefaciens-mediated transformation and shoot tip grafting in vitro regeneration system. We now report on the optimization of the cocultivation, regeneration and selection conditions for efficient and reliable production of transgenic lime (C. aurantifolia Swing.) plants. Improved transformation frequencies were obtained by cocultivating the explants with Agrobacterium on feeder plates. Optimum regeneration of transgenic shoots was obtained by exposing the explants to darkness for 2 weeks and by using kanamycin at 100 mg/l as selective agent. Attempts to use geneticin as selection antibiotic were not successful. Shoot tip grafting of regenerated shoots on Troyer citrange seedlings resulted in 100% successful production of transgenic plants. The presence and expression of the transferred genes in the regenerated plants was verified by β-glucuronidase histochemical and fluorimetric assays, neomycin phosphotransferase ELISA assays, PCR and Southern analyses.</div>
</front>
</TEI>
<affiliations><list><country><li>Espagne</li>
</country>
</list>
<tree><country name="Espagne"><noRegion><name sortKey="Pena, L" sort="Pena, L" uniqKey="Pena L" first="L." last="Pena">L. Pena</name>
</noRegion>
<name sortKey="Cervera, M" sort="Cervera, M" uniqKey="Cervera M" first="M." last="Cervera">M. Cervera</name>
<name sortKey="Juarez, J" sort="Juarez, J" uniqKey="Juarez J" first="J." last="Juarez">J. Juarez</name>
<name sortKey="Navarro, A" sort="Navarro, A" uniqKey="Navarro A" first="A." last="Navarro">A. Navarro</name>
<name sortKey="Navarro, L" sort="Navarro, L" uniqKey="Navarro L" first="L." last="Navarro">L. Navarro</name>
<name sortKey="Pina, J A" sort="Pina, J A" uniqKey="Pina J" first="J. A." last="Pina">J. A. Pina</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003371 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003371 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     Pascal:97-0468277
   |texte=   Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration

Genetic transformation of lime (Citrus aurantifolia Swing.) : factors affecting transformation and regeneration

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri